| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Chattanooga FC Academy Chattanooga FC - 2010B White |
0316-022B3U-7906 | Ryan Kelly |
| A2 | Kings Hammer KHM 10B Premier I |
0515-012B3U-0168 | Erich Delfs |
| A3 | Tri-Cities United Soccer Club Tri-Cities United 2010 Boys Premier |
0106-011B3U-2243 | O Chirica |
| A4 | Tennessee United Soccer Club TUSC 2010 Boys Red |
0424-012B3U-0013 | Helmut Liess |
| A5 | Knoxville FC (Crush) Knoxville FC Crush 2010B Premier |
0206-012B3U-5557 | Adelmo Santos |
| A6 | Chattanooga Red Wolves Academy Chattanooga Red Wolves Academy - 10B Red |
0324-022B3U-0043 | Owen Click |