| Group | Club/Team Name | Team ID | Head Coach |
| A2 | Chattanooga FC Academy Chattanooga FC - 2009B Navy |
0316-022B4U-7883 | B Wilkinson |
| A3 | Emerald Force Soccer Club - 01 Emerald Force 09 Manchester City |
0214-012B4U-1246 | Robert Gibson |
| A4 | Johnson City Futbol Club - 01 JCFC 2009B |
0216-012B4U-0104 | Dan Trull |
| A5 | Kings Hammer KHM 09B Premier II |
0515-012B4U-0164 | Christopher Dial |
| A6 | Knoxville FC (Crush) Knoxville FC Crush 09 B Premier |
0206-012B4U-0130 | Jorge Valdivia |
| A7 | FC Alliance Select Plus Teams FC Alliance 09/10B State League |
0204-031B4U-0137 | Michael Larson |
| A8 | Chattanooga Red Wolves Academy Chattanooga Red Wolves Academy - 09B Red |
0324-022B4U-0044 | James Lessentine |
| A9 | Blount United Soccer Club Blount United Soccer Club - 14U Boys Blue |
0201-012B4U-0231 | Thomas McCallum |