| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Nashville United Soccer Academy - 01 NUSA B08 Maroon |
0534-011B5U-0009 | Tommy Cochran |
| A2 | Tennessee United Soccer Club TUSC 2008 Boys NPL |
0424-011B5U-0008 | Miguel Arriaga |
| A3 | Lobos Rush (Collierville Soccer Association) 2008 Lobos Rush E64 |
0603-011B5U-0047 | Brandon Carter |
| A4 | Pateadores Soccer Club - Nashville Pateadores 08 Yellow |
0500-01D1B5U-0045 | Julio Hernandez |
| A5 | TN Tempo FC Academy Legacy Premiere Futbol Academy - 01 - B15U |
0592-011B5U-0004 | Sunebari Ndam |
| A6 | Pateadores Soccer Club - Nashville Pateadores 08 Blue |
0500-01D1B5U-0044 | Juan Carlos Garcia |
| A7 | Knoxville Reds Football Club - 01 Knox Reds 08 Celtic |
0290-01D1B5U-0003 | Derrick Long |
| A8 | Stones River Futbol Club - 01 Stones River FC 2008 Black |
0521-011B5U-2077 | Tommy Holt |