| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Lobos Rush (Collierville Soccer Association) 2007 Lobos Rush E64 |
0603-011B6U-0027 | David McAlister |
| A2 | Tennessee United Soccer Club TUSC 2007 Boys NPL |
0424-011B6U-0009 | Miguel Arriaga |
| A3 | Chattanooga Red Wolves Academy Chattanooga Red Wolves Academy - 07B Red |
0324-021B6U-0046 | Christopher Zeller |
| A4 | Emerald Force Soccer Club - 01 Emerald Force 07 Atletico |
0214-011B6U-4580 | Paul Bankston |
| A5 | Stones River Futbol Club - 01 Stones River FC 2007 Black |
0521-011B6U-2078 | B Ortega |
| A6 | Nashville United Soccer Academy - 01 NUSA B07 Maroon |
0534-011B6U-0010 | Adam Harris |
| A7 | Germantown Legends Germantown Legends 07 Black |
0606-011B6U-0007 | Kirk Dunaway |
| A8 | Germantown Legends Germantown Legends 07 Premier |
0606-011B6U-0008 | Justin Rose |