| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Tennessee United Soccer Club TUSC 2007 Boys Red |
0424-012B6U-0015 | Riley Sheen |
| A2 | ALBION SC Nashville (Formerly Southern Premier Soccer) Albion SC Nashville B07 Academy |
0540-02D3B6U-0022 | Brent Goulet |
| A3 | Clarksville Soccer Club Clarksville SC - B2007 Maroon |
0422-012B6U-0013 | Steven Young |
| A4 | Tennessee Soccer Club TSC Williamson 2007 Boys Showcase |
0522-011B6U-0205 | James Pollock |
| A5 | Germantown Legends Germantown Legends 07 White |
0606-012B6U-0018 | William Storey |
| A6 | Tennessee Soccer Club TSC Williamson 2007 Boys Premier |
0522-012B6U-0013 | Peter Russotto |
| A7 | Midsouth Futbol Midsouth Futbol Boys 2007 Elite |
0654-012B6U-0005 | Walter Aeschliman |
| A8 | Nashville United Soccer Academy - 01 NUSA B07 Navy |
0534-012B6U-0022 | F Charles |
| A9 | Kononia Soccer Club - 01 MIFC 07 |
0611-012B6U-1982 | Axel Sandoval |