| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Chattanooga Red Wolves Academy Chattanooga Red Wolves Academy - 06B Red |
0324-021B7U-0047 | Kuda Nhiwatiwa |
| A2 | Tennessee United Soccer Club TUSC 2006 Boys NPL |
0424-011B7U-0010 | Nate Sego |
| A3 | Clarksville Soccer Club Clarksville SC - B2006 Maroon |
0422-011B7U-0014 | Scott Dollar |
| A4 | Tennessee Soccer Club TSC Murfreesboro 2006 Boys Showcase |
0522-011B7U-0135 | Jason Finley |
| A5 | Germantown Legends Germantown Legends 06 Black |
0606-011B7U-0009 | A Carbognani - DO NOT USE |
| A6 | Tennessee Select Soccer Academy Tennessee Select Soccer Academy - B 06 |
0213-01CB7U-0015 | Bobby Deets |
| A7 | Jackson Soccer Club JSC 2006 Boys |
0609-012B7U-0006 | Malang Jarju |
| A8 | Stones River Futbol Club - 01 Stones River FC 2006 Black |
0521-011B7U-2079 | Jun Taulbee |
| A9 | Midsouth Futbol Midsouth Futbol Boys 2006 Premier |
0654-011B7U-0001 | Carl Schmitt |
| A10 | Nashville United Soccer Academy - 01 NUSA B06 Maroon |
0534-011B7U-0011 | Ethan Keogh |