| Group | Club/Team Name | Team ID | Head Coach |
| A1 | FC Alliance Select Plus Teams FC Alliance 04/05B State League |
0204-031B9U-0135 | Saahil Kapoor |
| A2 | Play Where You Stay - 01 PWYS '04 Boys |
0653-011B9U-0003 | Maximiliano Galizzi |
| A3 | Lobos Rush (Collierville Soccer Association) 2004/05 Lobos Rush E64 |
0603-011B9U-0054 | Carlos Rivera |
| A4 | Tennessee Soccer Club TSC Murfreesboro 2004 Boys Showcase |
0522-011B9U-0146 | Christian Dale |
| A5 | ALBION SC Nashville (Formerly Southern Premier Soccer) Albion SC Nashville B04 Academy |
0540-02D2B9U-0023 | Brent Goulet |
| A6 | Tri-Cities United Soccer Club Tri-Cities United 04/05 Boys Premier |
0106-011B9U-2237 | Nicolas Boegemann |
| A7 | Stones River Futbol Club - 01 Stones River FC 2004 Black |
0521-011B9U-2080 | Jun Taulbee |
| A8 | Chattanooga FC Academy Chattanooga FC - 2004/05B Navy |
0316-021B9U-7889 | Alexander Harrison |
| A9 | Nashville United Soccer Academy - 01 NUSA B04 Maroon |
0534-011B9U-0013 | John Sintic |
| A10 | Jackson Soccer Club JSC 2004 Boys |
0609-012B9U-0005 | Musa Manneh |
| A11 | Nashville United Soccer Academy - 01 NUSA B04 Navy |
0534-012B9U-0025 | Mirgen Bushati |