| Group | Club/Team Name | Team ID | Head Coach |
| A1 | FC Alliance ChallengeTeams FC Alliance 2012G Pre ECNL RL |
0204-02SG1U-0211 | Laban Defriese |
| A2 | Nashville United Soccer Academy - 01 NUSA G11 Maroon |
0534-01AG2U-0039 | Ethan Keogh |
| A3 | Knoxville Reds Football Club - 01 Knox Reds 11 Liverpool |
0290-01SJG2U-0006 | Cameron Broome |
| A4 | Chattanooga FC Academy Chattanooga FC - 2011G White |
0316-029G2U-7894 | Ron Senowech |
| A5 | Tennessee Soccer Club TSC Williamson 2012 Girls Academy Premier White - |
0522-01AG2U-2126 | Jason Mazuk |
| A6 | Tennessee United Soccer Club TUSC 2011 Girls Blue |
0424-01AG2U-0042 | Brianna Cole |