| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Chattanooga FC Academy Chattanooga FC - 2010G Navy |
0316-02GG3U-7896 | Juan Hernandez |
| A2 | Tennessee United Soccer Club TUSC 2010 Girls DPL |
0424-011G3U-0001 | Eduardo Carvacho |
| A3 | Nashville United Soccer Academy - 01 NUSA G10 Maroon |
0534-011G3U-0001 | Ali Arman |
| A4 | Tennessee Soccer Club TSC 2011 Girls Showcase Blue |
0522-011G3U-0041 | Dalton Kaufman |
| A5 | Tennessee Soccer Club TSC 2010 Girls ECNL |
0522-011G3U-0072 | Dean Blain |
| A6 | Tennessee Soccer Club TSC 2010 Girls Nike |
0522-011G3U-0071 | Lance Mayes |
| A7 | Knoxville Reds Football Club - 01 Knox Reds 10 Roma |
0290-01D1G3U-0001 | Julia French |
| A8 | Lobos Rush (Collierville Soccer Association) 2010 Lady Lobos Rush E64 |
0603-011G3U-0005 | Collin Scott |