| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Gallatin Rangers GSC Lady Rangers 10 |
0408-023G3U-0062 | Jordan Sharp |
| A2 | Kings Hammer KHM 10G Premier I |
0515-01BG3U-0166 | Serkan Orsan |
| A3 | Knoxville FC (Crush) Knoxville FC Crush 2010G Premier |
0206-012G3U-5556 | Josiah MacMillan |
| A4 | Nashville United Soccer Academy - 01 NUSA G10 Navy |
0534-012G3U-0014 | Ryan Burton |
| A5 | Wilson United Soccer Club WUSC - 2010 Girls Black |
0406-012G3U-0002 | Scott Davidson |