| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Armada Futbol Club Armada FC 09G Blue |
0436-012G4U-0055 | Kaitlynn Heileman |
| A2 | Tennessee Soccer Club TSC Williamson 2009 Girls Showcase |
0522-012G4U-0156 | Jenger Parrish |
| A3 | Chattanooga FC Academy Chattanooga FC - 2009G Navy |
0316-02DG4U-7897 | Scott Chapman |
| A4 | Knoxville FC (Crush) Knoxville FC Crush 09 G Premier |
0206-012G4U-0121 | James Thomas |
| A5 | Nashville United Soccer Academy - 01 NUSA G09 Gold |
0534-013G4U-0026 | David Vazquez |
| A6 | Nashville United Soccer Academy - 01 NUSA G09 Navy |
0534-012G4U-0015 | Stephanie Wallace |
| A7 | Tri-Cities United Soccer Club Tri-Cities United 09/10 Girls Premier |
0106-011G4U-2239 | Alex Butorovich |
| A8 | Chattanooga FC Academy Chattanooga FC - 2009/10G White |
0316-024G4U-7898 | Gary Smith |
| A9 | Tennessee United Soccer Club TUSC 2009 Girls Red |
0424-012G4U-0012 | Jeffrey Merry |