| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Tennessee United Soccer Club TUSC 2009 Girls DPL |
0424-011G4U-0002 | Andrew Fish |
| A2 | Knoxville Reds Football Club - 01 Knox Reds 09 Ajax |
0290-01D1G4U-0002 | Derrick Long |
| A3 | Tennessee Soccer Club TSC Murfreesboro 2009 Girls Showcase |
0522-012G4U-0155 | B Lewis |
| A4 | Armada Futbol Club Armada FC 09G Red |
0436-011G4U-0054 | Mark Champion |
| A5 | Kononia Soccer Club - 01 Kononia Lady Fire |
0611-011G4U-1980 | Sean Cogan |
| A6 | Lobos Rush (Collierville Soccer Association) 2009 Lady Lobos Rush E64 |
0603-011G4U-0006 | David McAlister |
| A7 | Nashville United Soccer Academy - 01 NUSA G09 Maroon |
0534-011G4U-0002 | Adam Harris |
| A8 | Kings Hammer KHM 09G Premier I |
0515-01BG4U-0170 | Alan Oates |
| A9 | Soccer Ole OLE FC 09G Premier |
0632-021G4U-0016 | Robby Midyett |